ABSTRACT
IgG isolated and purified from a healthy human serum through Sephadex G-200 and protein A CL 4B sepharose chromatography was used for detection of its own antibodies and correlated with the antiamoebic antibody titres in amoebic liver abscess cases. The mean titres with standard deviation of the self reactive antibodies to serum IgG both ALA cases and healthy controls show a highly significant difference, and antiamoebic antibody titres (IgG) are very much correlated with the autoreactive anti IgG titres in amoebic liver abscess cases. This correlation suggests that as antiamoebic IgG levels reach to its maximum, autoreactive anti IgG are produced to switch off antiamoebic anti IgG production in amoebic liver abscess cases.
Subject(s)
Animals , Antibodies, Anti-Idiotypic/biosynthesis , Antibodies, Protozoan/biosynthesis , Entamoeba histolytica/immunology , Humans , Immunoglobulin G/blood , Liver Abscess, Amebic/immunologyABSTRACT
Testes para citotoxicidade e lise amebiana foram utilizados para deminstrar uma possível interaçäo entre o fator reumatóide e a Entamoeba histolytica. A atividade citotóxica amebiana foi inibida pela IgG antiameba de coelho purificada através de cromatografia. Constatou-se inibiçäo aumentada com IgG antiameba de coelho mais fator reumatóide. A mesma inibiçäo acentuada da atividade citotóxica amebiana pôde ser constatada quando se substituiu o fator reumatóide por sor humano normal, inativado pelo calor, como controle. Cerca de 50% de lise amebiana ocorreu quando as amebas foram misturadas com soro normal humano como fonte de complemento. A lise amebiana aumentou para 60% quando incubadas com soro humano normal, acrescido de anticorpos humanos antiameba. Nenum aumento adicional pode ser obtido pela adiçäo de fator reumatóide. Usando IgG antiameba de coelho em vez de anticorpos humanos, a proporçäo de lise näo aumentou. A incubaçäo de amebas com soro humano normal, IgG antiameba de coelho e fator reumatóide reduziu acentuadamente a lise amebiana. O fator reumatóide näo teve efeito na atividade citotóxica amebiana, nem na lise amebiana mediada pelo complemento in vitro
Subject(s)
Humans , Animals , Antibodies, Anti-Idiotypic/biosynthesis , Entamoeba histolytica/immunology , Immunoglobulin G/biosynthesis , In Vitro Techniques , Rheumatoid Factor/biosynthesis , Killer Cells, Natural/immunology , Chromatography, Affinity , Cytotoxicity, Immunologic/immunology , Cytotoxicity Tests, ImmunologicABSTRACT
Anti-human globulin was produced in local rabbits and goats by using human O serum mixed with Freund's adjuvants (complete or incomplete) for immunising the animals. A titre of 1/1024 was obtained in rabbit and a titre of 1/8192 was obtained in goat. Quality-and quantity-wise, goat's serum appeared to be better than rabbit's serum.